To find out the optimum pH at which the enzyme catalase would best break down hydrogen peroxide.

GCSE SCIENCE COURSEWORK (BIOLOGY) PLANNING: Skill Area P spell down the level of your Investigation. The aim of investigation is that to find issue the optimum pH at which the enzyme catalase would opera hat amend down hydrogen peroxide. Briefly describe your advance experimentation and include your results. Explain how these results involve helped you plan your of import trial (p8b). The purpose of the preliminary experiment was to find out the trump out stemma of catalase for the investigation. The quartette choices of sources which were available were liver, orchard apple tree, yeast and potato. to each 1 source was cut up into 1cm3 sections and placed in cardinal separate evidence tubes which each contained 5ml of H2O2. Observations were made on the physical evidence of what happened. Catalase source         Degree of bubbling         Number of propagation relights radiancy splint potato         normal      Â Â Â Â 1 apple         little         0 liver         a lot         7 yeast         Quite a lot         3 As the result show above, the potato was considered to be the best source to be customd in the real experiment be fountain it findd enough oxygen (but not too much(prenominal) to cause a dangerous situation). The apple did not turn up to have sufficient catalase for the experiment as it did not produce each oxygen. The yeast and the liver, while rich in catalase regrettably prove to be over sufficient with an unnecessarily overlarge make out of the enzyme needed. The oxygen is produced by this equation: describe all the apparatus that you at once plan to use (diagrams should be included) (P4b).

20ml of each buffer issue from pH3 to pH8 120ml of H2O2                                                      6 test tubes Three medium sized raw potatoes                           one bob borer 50ml Manometer fluid                                    2x syringes 1x Ruler                                                      2x Pipettes 1x White tile                   ... If you necessity to get a full essay, order it on our website: BestEssayCheap.com

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